Fig. 1

DNA sequence recognition by the C2H2-ZF domain. a Schematic representation of C2H2-ZF structure and interaction with DNA. The base-contacting residues (blue) interact with three or four DNA bases. The eight non-base-contacting residues of the zinc finger (bounded by C and H) are shaded gray. Arrows, loop, and helix in the schematic illustration at the top denote the beta-sheets, turn, and alpha helix structures, respectively, with the arrowheads pointing toward the protein C-terminus. b Example of motifs obtained from individual C2H2-ZF domains using B1H [16] and comparison to the motif obtained from full-length protein using SELEX [45]. c Neighboring C2H2-ZFs can interact with each other and affect the orientation of binding, resulting in multiple binding modes depending on the residues at the interface of the interaction. Figure is adapted from ref [7]