Fig. 6

Analysis of homologous pairing. a Schematic view of surface-to-surface distances between homologous domains. Different domains exhibit different degrees of homolog pairing. b Left panel: Pairing frequency for each euchromatin domain, plotted by chromosome. We define a domain as being paired in a structure if the surface-to-surface distance between the two homologs is less than 200 nm. The x-axes are the same as the plots in Fig. 5a. The domains are colored by their epigenetic classes: green, HP1; blue, PcG; black, null; red, active. Right panel: Density plots of the domain pairing frequencies, grouped by epigenetic class. The active class has the smallest mean homologous pairing frequency for each chromosome. c Reproducibility of the average homolog distances between two independently generated structure populations. The Pearson’s correlation between them is 0.998, with p value <2.2e − 16. d The correlation between the pairing frequencies of homologous domains and their Mrg15 enrichment is negative. The Mrg15 scores range from 0.8 to 3.0 and are divided into 21 equal bins. The corresponding pairing frequencies from our models in a given Mrg15 bin are summarized as a mean and variance, and the latter is displayed as an error bar. The blue dashed line is the linear regression between the average pairing frequency in each bin and the midpoint Mrg15 enrichment value of the bin. The Pearson’s correlation between them is −0.81, with p value = 7.59-e − 06. e Left panel: Pairing frequencies of homologous domains grouped by epigenetic class. Right panel: Enrichments of Mrg15 binding grouped by epigenetic class. Active domains are generally more enriched with Mrg15, and have lower pairing frequencies, than the other three repressive classes