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Fig. 2 | Genome Biology

Fig. 2

From: The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana

Fig. 2

Depletion of DNA methylation over gene bodies and H3.3-enriched regions in h3.3kd. ad Genome-wide BS-seq results showing enrichment profiles of DNA methylation in WT, h3.3kd-2, and h3.3kd-3. a CG DNA methylation patterns over genes in WT plants. Genes were aligned from transcription start site (TSS) to transcription end site (TES) and grouped into quintiles according to their level of expression. b Relative CG methylation levels over gene bodies in h3.3kd compared to WT. Note pronounced loss of methylation at the 3′ ends of highly and moderately expressed genes. c, d DNA methylation levels in all contexts (CG, CHG, and CHH) over H3.3-enriched regions (c) and TEs (d) in WT and h3.3kd. e Expression of DNA methylation-related factors in WT compared to h3.3kd-3 (RNA-seq)

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