Fig. 2

Mutation dynamics over many cycles of MAGE allele reversion. a Increase in combinatorial diversity and reversion count vs. number of MAGE cycles. b Number of reversions per clone vs. MAGE cycle. c The rate of reversions per MAGE cycle among the different allele categories, showing a higher rate per cycle for cells exposed to all 127 oligos. d The number of de novo mutations per clone over successive MAGE cycles. e Rate of de novo mutations per MAGE cycle. f The average ratio between number of de novo mutations and reverted alleles per MAGE cycle remains constant throughout the experiment. g Doubling time (min) improvement per clone from the C321.∆A starting strain (top dotted line) towards the ECNR2 parent strain (bottom dotted line). Blue line is a LOESS fit