Fig. 2
From: SCALE: modeling allele-specific gene expression by single-cell RNA sequencing
Overview of analysis pipeline of SCALE. SCALE takes as input allele-specific read counts at heterozygous loci and carries out three major steps: (i) gene classification using an empirical Bayes method, (ii) estimation of allele-specific transcriptional kinetics using a Poisson-Beta hierarchical model with adjustment of technical variability and cell size, (iii) testing of the two alleles of a gene to determine if they have different bursting kinetics and/or non-independent firing using a hypothesis testing framework