Fig. 1
From: Single-cell transcriptome conservation in cryopreserved cells and tissues
Comparative analyses of MARS-Seq-derived single-cell transcriptome data from fresh (red) and cryopreserved (–80 °C: blue; liquid nitrogen: green) HEK293 cells. a Mapping distribution of sequencing reads after MARS-seq library preparation. Each line represents a single cell and transcript sizes are scaled from 0 to 100. b Cumulative gene counts split by fresh and cryopreserved cells and analyzed using randomly sampled cells (average of 100 permutations). c, d Comparative analysis of the number of sequencing reads and detected transcripts (c) or genes (d) per cell using a linear model. The slope of the regression line was calculated separately for fresh and cryopreserved cells. e, f Gene expression profile variances between fresh and cryopreserved cells displayed as principal component analysis (PCA, e) or as t-distributed stochastic neighbor embedding (t-SNE) representation (f) using the 100 most variable genes