Fig. 4

Assessment of the IMP-based iterative co-assemblies in comparison to MOCAT- and MetAMOS-based co-assemblies. Radar charts summarizing the characteristics of the co-assemblies generated using IMP, MetAMOS, and MOCAT pipelines on: a human fecal microbiome, b wastewater sludge community, c biogas reactor, d simulated mock community. IMP co-assemblies were performed with two de novo assembler options, IDBA_UD and MEGAHIT, whereas MetAMOS and MOCAT were executed using default settings. Assessment metrics within the radar charts include number of contigs ≥1 kb, N50 length (contiguity, cutoff 500 bp), number of predicted genes (unique), and fraction of properly mapped MG and MT read pairs. N50 statistics are reported using a 500-bp cutoff. Additional ground truth assessments for simulated mock dataset included recovered genome fractions (%) and the composite performance metric (CPM) score with a cutoff of 500 bp [62]. e Summary radar chart reflecting the cumulative measures and mean fraction of properly mapped MG and MT read pairs from all analyzed 11 datasets while incorporating ground truth-based measures from the simulated mock dataset. Higher values within the radar charts (furthest from center) represent better performance. Detailed information on the assembly assessments is available in Additional file 2: Table S5