Fig. 6

Hypo-enhancers interact with target gene promoters throughout development. a Distribution of distances from viewpoints of all non-redundant 4C interactions observed. The five viewpoints analyzed by 4C-Seq were pooled. b Overlap between all non-redundant 4C interaction sites and ChIP-Seq peaks observed and expected by chance as indicated (*p < 2.2 × 10⁻¹⁶, non-paired Wilcoxon-test). c, d 4C-Seq profiles generated at 8 and 24 hpf and from brain. Red vertical arrows under every 4C-Seq panel indicate the viewpoint. All significant 4C interactions are indicated by blue rectangles below every 4C-Seq panel and 4C interactions with a TSS are highlighted in orange. Below the 4C-Seq panels, tracks indicating hypo-enhancers (purple) and ChIP-Seq peaks (H3K4me1, red; H3K27ac, black) throughout development are shown. To the right of the 4C-Seq panels we depict the expression of the indicated genes at the indicated developmental time points or tissue