Fig. 1

Non-canonical splicing in Globodera. a Correction of many exon-intron boundaries to be consistent with RNA-seq mapping required the use of a non-canonical 5′ donor site. Comparison of the consensus sequence for both canonical (GT/AG) and non-canonical (GC/AG) splice sites reveals similar local base composition, with the exception of the GT or GC itself. b The 5′ donor sites of both GC/AG and GT/AG introns conform to the consensus CAGG[T|C]AAGT. c GC/AG introns are less common at the beginning of gene models in both G. rostochiensis (black) and G. pallida (grey)