Fig. 4

Comparison of invFBA predictions and metabolite secretion. Metabolite secretion flux (full line), inferred from experimental data through the TEAM approach, and maximum coefficient of the secretion flux in the objective function (dashed line), as predicted by invFBA (through OVA), are plotted as a function of time. Metabolite and gene expression data come from time-dependent measurements performed during batch aerobic growth of the bacterium S. oneidensis on lactate. The secreted metabolites are pyruvate in (a), glycolate in (b), and acetate in (c). Positive fluxes reflect secretion of the metabolite in question, while negative fluxes reflect uptake