Fig. 2
From: Pooling across cells to normalize single-cell RNA sequencing data with many zero counts
Illustration of the effect of removing stochastic zeroes (black) from the distribution of ratios across all genes. Distributions are shown for cells with a small and b large θ j . The estimated median ratio (dashed) is increased beyond the true median (full) upon removal of zeroes, which results in overestimation of the size factor for the cell. This effect is more pronounced for cells with small θ j that have greater numbers of zeroes, compared to cells with large θ j where the estimated and true medians are more similar