Single-cell sequencing in stem cell biology

Table 3 Summary of single-cell genome sequencing technologies

Assays	Strategies	Principles	Reference(s)
DOP-PCR	Degenerate oligonucleotide-primed PCR	Exponential	[90]
MDA	Phi 29 DNA polymerase-based MDA	Exponential	[91]
MALBAC	Multiple annealing and looping-based amplification cycles	Quasi-linear	[92]
Microfluidic MDA	MDA in a microfluidic chamber	Exponential	[93]
MIDAS	MDA in hundreds to thousands of nanoliter wells	Exponential	[94]
eWGA	MDA in millions of picoliter droplets	Exponential	[95]
Nuc-seq	MDA for single cells in S phase	Exponential	[96, 97]

DOP degenerate oligonucleotide-primed, eWGA emulsion whole-genome amplification, MALBAC multiple annealing and looping-based amplification cycles, MDA multiple displacement amplification, PCR polymerase chain reaction

ISSN: 1474-760X