Skip to main content


Table 3 Summary of single-cell genome sequencing technologies

From: Single-cell sequencing in stem cell biology

Assays Strategies Principles Reference(s)
DOP-PCR Degenerate oligonucleotide-primed PCR Exponential [90]
MDA Phi 29 DNA polymerase-based MDA Exponential [91]
MALBAC Multiple annealing and looping-based amplification cycles Quasi-linear [92]
Microfluidic MDA MDA in a microfluidic chamber Exponential [93]
MIDAS MDA in hundreds to thousands of nanoliter wells Exponential [94]
eWGA MDA in millions of picoliter droplets Exponential [95]
Nuc-seq MDA for single cells in S phase Exponential [96, 97]
  1. DOP degenerate oligonucleotide-primed, eWGA emulsion whole-genome amplification, MALBAC multiple annealing and looping-based amplification cycles, MDA multiple displacement amplification, PCR polymerase chain reaction