Single-cell sequencing in stem cell biology

Table 2 Summary of single-cell epigenome sequencing technologies

Epigenetic marks	Assays	Strategies	Coverage	Reference
5mC	scRRBS	RRBS	0.5–2 M CpG sites	[74]
5mC	scBS	PBAT	0.5–10 M CpG sites	[75]
5mC	scWGBS	PBAT-like	0.5–10 M CpG sites	[76]
Chromatin accessibility	scATAC-seq	ATAC-seq	Average 73,000 unique fragments mapping to genome	[84]
Chromatin accessibility	scATAC-seq	ATAC-seq	50–6000 DHS sites	[85]
Chromatin accessibility	scDNase-seq	DNase-seq	Average 317,000 unique fragments and 38,000 DHS	[86]
Chromatin structure	Single-cell Hi-C	Hi-C	Not available	[87]
Chromatin structure	Single-cell DamID	DamID	Not available	[88]
Histone modification	Drop-ChIP	Droplet-based ChIP-seq	1000 H3K4me2 peaks	[89]

5mC 5-methylcytosine, ATAC assay for transposase-accessible chromatin, BS bisulfite sequencing, ChIP chromatin immunoprecipitation, DamlD Dam identification, DHS DNase I hypersensitive sites, MALBAC multiple annealing and looping-based amplification cycles, PBAT post-bisulfite adaptor tagging, RRBS reduced representation bisulfite sequencing, sc single-cell, WGBS whole-genome bisulfite sequencing

ISSN: 1474-760X