Skip to main content
Fig. 5 | Genome Biology

Fig. 5

From: In silico lineage tracing through single cell transcriptomics identifies a neural stem cell population in planarians

Fig. 5

In situ validation of candidate neural lineage genes identified with Waterfall. a Colorimetric WISH. Dorsal view, anterior up, scale bars = 100 μm. b Pseudotime plots for genes in a. Red line, local polynomial regression fit; shaded region, 95 % confidence interval. c Diagram of the region imaged (red box) in d–f. d tFISH of each ν-gene with piwi-2 and piwi-1. Boxed regions are magnified and displayed with DAPI below each image. e dFISH of each ν-gene with piwi-2 and immunofluorescence for PIWI-1. Boxed regions are magnified and displayed with DAPI below. f dFISH of each ν-gene with piwi-2 and immunofluorescence for BrdU following injection and a 4-h chase period. Boxed regions are magnified and displayed below. The rightmost high magnification panels are merged images

Back to article page

Genome Biology

ISSN: 1474-760X

Contact us