Fig. 3
Pseudotime analysis with Waterfall predicts a neural lineage trajectory. a PCA plot for Groups 1 and 2 and νNeoblasts. Grey arrow, proposed lineage orientation; each point is a single cell. b PCA plots with data point size proportional to the expression level of the gene specified in each plot. c Plot of Groups 1 and 2 and νNeoblast cells ordered along pseudotime. Red line, linearized minimum spanning tree connecting k-mean centers; grey arrow, direction of lineage progression; y-axis, distance of cell to its nearest k-means center. d–g Expression patterns of known markers support the Waterfall lineage prediction: d differentiation gene mex3-1; e stem cell markers piwi-1, vasa-1, and HDAC-1; f cell cycle markers h2b, mcm-5, and cyclinB; g neural markers pc-2, chat, and ascl-1. Red line, local polynomial regression fit; shaded region, 95 % confidence interval