Fig. 3
From: KIAA0556 is a novel ciliary basal body component mutated in Joubert syndrome
KIAA0556 mutation in mice results in hydrocephalus. a Mouse with D430042O09Rik genetrap (KIAA0556 mutation; GT/GT) with severe hydrocephalus next to heterozygous littermate (WT/GT). b Nissl stained brain sections from genetrap and heterozygous mice showing ventricle enlargement (arrows) and compressed hippocampi in the mutant. c Brain slices from mice injected with Evans blue to trace fluid flow through the ventricles. The genetrap mouse has blockage in the cerebral aqueduct (arrows) revealed by a lack of blue dye. d Analysis of the frequency of ependymal cilia beating and ventricular fluid flow velocity in genetrap (GT) and control wild-type (WT) mice showing similar rates. Error bars; standard deviations. e Sections of the hippocampus in genetrap and control mice showing normal primary cilia, indicated by adenyl cyclase III staining. Scale bar, 20 μm