Fig. 2
From: Glucocorticoid receptor and nuclear factor kappa-b affect three-dimensional chromatin organization
P300 ChIA-PET interaction profile shows an enhancer-centered interaction pattern in contrast to promoter-centered RNA polymerase II (POLII) interactome. a Proportion of distal binding site (DBS)-promoter, promoter-promoter, and DBS-DBS interactions identified by P300 ChIA-PET in cells treated by vehicle (dimethyl sulfoxide [DMSO]) (upper panel) or triamcinolone acetonide (TA) + tumor necrosis factor alpha (TNFα) (lower panel). b Proportion of DBS-promoter, promoter–promoter, and DBS–DBS interactions identified by POLII ChIA-PET in cells treated with vehicle (upper panel) or TA + TNFα (lower panel). c Venn diagram depicting the extent of overlap between P300 interaction subdomains and POLII interaction subdomains (upper panel). Histogram depicting the percentage of P300 and POLII shared anchors in P300 & POLII interaction subdomains (lower panel). d Example screenshots of P300-rich (left panel), P300 & POLII-rich (middle panel), and POLII-rich (right panel) interaction subdomains depicting the ChIP-seq and ChIA-PET interaction data. e Distribution of glucocorticoid receptor (GR) (left panel) and p65 (right panel) binding sites in P300 rich, P300 & POLII-rich, and POLII-rich interaction subdomains