Fig. 3
Epigenetic clock CpGs co-localize with functional glucocorticoid response elements (GREs) and show methylation changes following GR activation. a Epigenetic clock CpGs co-localize with functional GREs. GRE peaks were derived from ENCODE NR3C1 ChIP-seq data from lymphoblastoid cell lines. Among the 353 epigenetic clock CpGs, 85 CpG sites were noted to be located within GR ChIP-Seq peaks in a lymphoblastoid cell line (shown with the red dotted line) (Additional file 1: Table S1). This number significantly differed (pperm <0.001) from the CpG-GRE overlap predicted by 1,000 randomly selected sets of CpGs covered by the 450 K array (mean 48.8, SD 6.14, range 31 to 68). b Epigenetic clock CpGs that are significantly regulated by DEX exposure are in proximity to GREs. GRE peaks were derived from ENCODE NR3C1 ChIP-seq data from lymphoblastoid cell lines. Volcano plot was zoomed for +/− 10 kb distance around the GRE peaks. The dotted red line in the volcano plot represents the level of statistical significance (P = 5 × 10−2) after FDR correction for multiple comparisons. Further details on DEX-regulated CpGs are given in Additional file 1: Table S1