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Fig. 1 | Genome Biology

Fig. 1

From: P16-specific DNA methylation by engineered zinc finger methyltransferase inactivates gene transcription and promotes cancer metastasis

Fig. 1

P16-specific methyltransferase (P16-dnmt) induces methylation of P16 CpG islands and represses gene transcription in HEK293T cells. a Construction of P16-dnmt using P16 promoter-specific seven-zinc finger protein (7ZFP) and the catalytic domain of mouse DNMT3a; b Western blot analysis for P16-Dnmt and endogenous P16; c Reporter assay results following P16-dnmt transfection; d DHPLC methylation analysis of the P16 promoter in HEK293T cells; the 392-bp methylated (M) and unmethylated (U) P16 fragments were detected at the partial denaturing temperature of 57.0 °C; e bisulfite clone-sequencing results from HEK293T cells transiently transfected with P16-dnmt

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Genome Biology

ISSN: 1474-760X

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