Fig. 1
From: Spatio-temporal regulation of circular RNA expression during porcine embryonic brain development
Features of cortical circRNAs. a, b The number of circRNAs expressed at various cutoff expression levels. c Cumulative plot showing length of introns flanking circRNAs with expression levels categorized as either low (0.05 to 0.5 RPM, red line), medium (0.5 to 2.5 RPM, green line) or high (>2.5 RPM, purple line) compared with introns flanking exclusively linear spliced control exons (non-circRNA-forming internal exons from genes that do form circRNAs at other exons, black line). Median intron lengths are shown. Introns of all three circRNA subgroups are significantly larger than the control. d The intron groups from (c) examined for non-complementary and complementary short interspersed nuclear elements (SINEs) within the first 500 bp of flanking introns. e A cumulative plot of the distance between complementary SINE pairs in flanking introns of the intron groups from (c). The distance between flanking SINEs is the total genomic distance minus the distance between splice sites involved in circularization. The median distances between complementary SINE pairs are shown. f The percentage of human and mouse circRNAs with identical counterparts in embryonic pig cortex after use of UCSC liftOver tools (blue) compared with random in silico-generated control circRNAs (black). P values: *P < 10−5, **P < 10−10, ***P < 10−20. ESC embryonic stem cell, SK-N-SH RA SK-N-SH neuroblastoma cells differentiated by retinoic acid