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Table 1 Top ten genes originally calculated using S/N (for edgeR-normalized data; first ten rows) and top ten genes calculated using FDR for DE (edgeR P values; second ten rows). The table includes the counts-per-million table (A = GM12892 and B = H1-hESC), S/N and estimated false discovery rate (FDR) for edgeR and limma-voom for the ENCODE dataset comparing three replicates of GM12892 to four replicates of H1-hESC

From: Do count-based differential expression methods perform poorly when genes are expressed in only one condition?

Id

A1

A2

A3

B1

B2

B3

B4

edgeR S/N

edgeR FDR

voom S/N

voom FDR

MIPOL1

0.0

0.0

0.0

237.1

232.5

226.0

227.5

45.75

7.47e −31

3.79

3.32e −07

AQP4

0.0

0.0

0.0

46.1

45.0

46.7

44.4

43.87

1.44e −14

3.90

1.99e −06

FAM19A4

0.0

0.0

0.0

142.1

131.1

143.8

131.4

20.21

1.91e −24

4.06

4.72e −07

C17orf66

2.3

2.1

2.1

0.0

0.0

0.0

0.0

16.96

1.89e −02

2.72

7.40e −04

TM4SF19

3.5

3.2

3.2

0.0

0.0

0.0

0.0

16.96

3.00e −03

2.72

2.39e −04

SOX1

0.0

0.0

0.0

7.5

6.7

6.5

6.3

13.02

8.37e −05

4.33

1.27e −04

HPGD

0.0

0.0

0.0

22.6

21.0

19.6

19.0

12.97

5.08e −09

4.56

7.48e −06

LOC100131176

0.0

0.0

0.0

17.9

15.3

18.7

17.2

12.02

5.14e −08

3.51

1.49e −05

ZNF385D

0.0

0.0

0.0

135.5

155.0

155.0

132.3

11.80

2.60e −25

4.67

3.70e −07

NPY1R

0.0

0.0

0.0

209.8

179.9

179.3

208.5

11.38

1.33e −27

2.95

6.77e −07

PLEK

25 082.8

12 622.5

11 394.8

0.0

0.0

0.0

0.0

2.16

1.79e −216

16.11

9.36e −09

MS4A1

25 455.1

14 937.7

12 886.8

0.0

0.0

0.0

0.0

2.63

2.62e −215

7.26

1.60e −08

SLAMF1

7 407.2

4 859.3

4 283.2

0.0

0.0

0.0

0.0

3.32

2.98e −165

5.11

2.53e −08

CCL3

11 057.5

3 413.1

3 544.1

0.0

0.0

0.0

0.0

1.37

4.62e −165

5.13

2.15e −08

FCRLA

7 742.0

2 979.1

3 879.8

0.0

0.0

0.0

0.0

1.92

1.01e −161

6.08

1.84e −08

RGS1

9 939.5

9 967.3

7 741.6

0.0

0.0

0.0

0.0

7.22

4.53e −159

2.66

1.44e −07

DPPA4

0.0

0.0

0.0

14 580.2

15 215.1

14 745.3

10 617.3

6.47

2.37e −158

12.02

1.84e −08

TDGF1

0.0

0.0

0.0

15 699.8

15 481.1

13 374.5

8 522.5

3.98

6.37e −157

15.48

1.84e −08

SFRP2

0.0

0.0

0.0

14 673.3

15 229.5

13 067.2

7 234.4

3.43

1.84e −153

9.87

2.15e −08

BLK

9 943.0

2 954.7

2 351.8

0.0

0.0

0.0

0.0

1.20

2.98e −147

3.28

5.17e −08