Fig. 2

HP1β regulates developmental genes and pluripotency factors in ESCs. a Number of misregulated genes in HP1α^−/− (empty bars) and HP1β^−/− (filled bars) ESCs and EBs compared with WT cells at a 0.005 (left) or 0.05 (right, shaded) confidence level, which correspond to 2.5- and 1.5-fold change, respectively. b Expression fold change in HP1α^−/− (empty bars) and HP1β^−/− (filled bars) ESCs compared with WT cells of developmental marker genes representative of endoderm (top), mesoderm (middle) and ectoderm (bottom). The shaded grey area represents fold changes which are not statistically significant. c Gene Ontology analysis of biological processes affected in the HP1β^−/− ESCs. Biological processes where bars in the graph go to the left of zero (here, only “regulation of cell proliferation”) are those affected significantly by genes downregulated in HP1β^−/− ESCs, whereas those where bars in the graph go to the right of zero are those significantly affected by genes upregulated in HP1β^−/− ESCs. The actual p values are shown for each biological process. The list of the misregulated genes was analyzed according to their functional annotation and the biological processes they belong using the Database for Annotation, Visualization and Integrated Discovery (DAVID). d Expression fold change in HP1α^−/− (empty bars) and HP1β^−/− (filled bars) ESCs compared with WT cells of key pluripotency factors. The grey area represents fold changes which are not statistically significant