Fig. 2
From: Extensive identification and analysis of conserved small ORFs in animals
Predicted sORFs are under purifying selection and often widely conserved. a Adjusted phyloCSF scores for predicted sORFs are higher than those from control sORFs matched by their nucleotide conservation level (phastCons). b The dN/dS ratio of SNPs for novel predicted sORFs is smaller than for control ORFs in non-coding regions of the transcriptome, but larger than for annotated sORFs. c Percentage of sORFs conserved in ancestral species as inferred from the multiple species alignment. Numbers for informative ancestors are indicated (for example, the ancestors of primates, placental mammals, and jawed vertebrates for H. sapiens). Symbols mark different reference species as in (d). d homology clustering of predicted sORFs in different species; only clusters with at least one non-annotated member and members from more than one species are shown, with multiplicity indicated. ***P <0.001; **P <0.01; *P <0.05; Mann-Whitney tests in a, reciprocal Χ2 tests in b