Graded gene expression changes determine phenotype severity in mouse models of CRX-associated retinopathies

Table 2 List of RNA-seq experiments and number of genes with altered expression

Mouse^a	Experiments and replicates^b		Down-regulated genes^c		Up-regulated genes^c
Mouse^a	P10	P21	P10	P21	P10	P21
WT	3	3	NA	NA	NA	NA
E168d2/d2	3	Not tested	425	NA	248	NA
R90W/W	3	Not tested	195	NA	70	NA
E168d2/+	3	3	136	150	61	43
E168d2neo/+	3	3	85	83	38	19
R90W/+	3	3	20	27	12	27

^a All mutant mice were backcrossed to C57BL/6 J (WT) control for >10 generations and genotyped for common variants [21]
^b Numbers represent biological replicates. Each replicate contains four pooled retinas from a male and female pair
^c Numbers represent transcripts significantly altered (FC ≥ 2, FDR ≤ 0.05) relative to WT control
NA not applicable

ISSN: 1474-760X