Fig. 1
From: Exploiting single-molecule transcript sequencing for eukaryotic gene prediction
Identification of full-insert cDNA sequences in SMRT sequencing data. Colors refer to the different types of sequences that can be encountered within the read data, that is 5’ and 3’ cDNA synthesis primers, PacBio SMRT library preparation adapter, and cDNA sequences consisting of 5’ UTR, open reading frame (ORF), 3’ UTR, and poly(A) tail. Initially, reads were subclassified into two groups: SMRT reads consisting of several subreads (left) or individual subreads (right). Reads from both groups were error-corrected and used to identify full-length cDNA sequences