Fig. 3

Qualitative identification of breakage and translocation events with hiBA-FISH. a Representative maximal projections of 40× confocal images of NPM1-ALK translocation-negative Mac2A cells, untreated or treated with 25 Gy of ionizing radiation, and untreated NPM1-ALK translocation-positive K299 cells stained with the hiBA-ALK probe set. Overlays of the three FISH probe channel images [19], the nucleus segmentation (yellow) and FISH spot detection (bottom). Magnification of an intact ALK allele event (i). Broken ALK allele events (ii). NPM1-ALK translocation events (iii and iv). Scale bar 10 μm. b Same as (a), but cells were stained with the hiBA-NPM probe set. Magnification of an intact NPM1 allele event (i). Broken NPM1 allele events (ii). NPM1-ALK translocation events ((iii and iv)). Scale bars 10 μm