Fig. 2

ROR modulates tumor growth and metastasis. a, b MTT assay showing tumor cell growth after ROR knock-down. Cell growth was obviously restrained at day 3 in ROR-silenced AGS and HT29 cells. The absorbance values were detected at 24 h, 48 h, and 72 h, the control was arbitrarily set at 100 % on day 1. c, d The migratory ability of ROR-silenced tumor cells. The ability to metastasize in ROR-silenced tumor cells was remarkably reduced compared with the untreated control cells. The 570 nm absorbance values of the control were set at 1. The migration detection was conducted at 24 h for AGS cells (c) and 48 h for HT29 cells (d). All of the data are presented as the mean ± SD. *P <0.05: compared with the control and mock. e Images of a soft agar tumor colony. Few visible colonies were observed in the ROR knockdown tumor cells. Bars: 100 μm. f Colony count statistics demonstrate tumor formation ability. Colony count statistics showed a significant reduction in ROR-silenced AGS and HT29 cells. Colony numbers were determined from three independent soft agar plates. All of the data are presented as the mean ± SD. *P <0.05: compared with the control and mock