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Table 1 Approximate cost of sequencing for each read length and sequencing type on a HiSeq 2500, high-output mode v3 (eight lanes per flowcell)

From: The impact of read length on quantification of differentially expressed genes and splice junction detection

  Per lane cost
Read configuration Single end Paired end
25 bp $950 $1,275
50 bp $1,100 $1,650
75 bp $1,250 $2,025
100 bp $1,400 $2,400