Fig. 2
Intra-tumoral heterogeneity of PDX cells. a Scatter plots of the average gene expression of single cells (H358, n = 50; LC-PT-45, n = 34; LC-PT-45-Re, n = 43) compared with those of the corresponding bulk cells (~1 × 105 cells). Black dotted lines are x = y lines with correlation coefficients (Pearson r and Spearman r) for linear fit. TPM transcripts per million. b Inter-correlation (Pearson r) between gene expression in single cells. Density plots were constructed with a kernel function fitting over the histograms. c Explanatory power (adjusted R-square) of gene expression in various numbers of single cells relative to the bulk cells was determined by multiple regression analysis with randomly selected cell numbers with permutation (×1000). d Overlap ratio of expressed single-nucleotide variations (SNV) among single cells. Density plots were constructed with a kernel function fitting over the histograms. e Overlap ratio of expressed SNVs of various numbers of single cells relative to that of the bulk cells was calculated with a randomly selected given number of cells with permutation (×1000). For boxplots in (c) and (e), box = interquartile range between the first and the third quartiles, error bars = 10th–90th percentiles