Fig. 7
From: Scalable microfluidics for single-cell RNA printing and sequencing
Cell type separation by single-cell RNA-Seq. a iPAGE gene ontology/pathway analysis based on rank-ordering of differentially expressed genes using +/−(1-p) where p is the P value for differential expression between the U87- and MCF10a-exclusive lanes given by the Wilcoxin rank-sum test. Values are positive for genes more highly expressed in U87 and negative for genes more highly expressed in MCF10a. b t-SNE clustering of 396 single-cell profiles based on the differentially expressed genes color-coated by the lane-of-origin of each profile. Two clear spatial clusters form and each is predominantly associated with a specific cell type-exclusive lane. c The same t-SNE clustering shown in (b) but color-coated with a score indicating expression of the U87-specific genes vs. the MCF10a-specific genes. The score is based on the relative rank ordering of U87- and MCF10a-specific genes in each cell (see Methods)