Fig. 2

Phenotypic importance of genes with consistent expression. a Enriched Gene Ontology biological process categories across commonly expressed genes. Category abbreviations: biosynth. biosynthetic; cmpd. compound; depolymer. depolymerization; deriv. derivation; dev. development; gen. generation; metab. metabolites; org. organization; oxid. oxidation; polymer. polymerization; prec. precursor; proc. process; reg. regulation; synth. synthesis. b Association of common gene expression (rows) and mutant phenotypes (columns). Mammalian Phenotype Ontology phenotypes were grouped for affecting brown adipose tissue (Adi), myocardial tissue (Car), or brain tissue (Brain), or causing prenatal lethality [47, 48] (Table S7 in Additional file 1). Circle size indicates enrichment of phenotypic category in commonly expressed genes. Bonferonni-corrected p values are included for significant chi-square tests. c, d Overlap of common genes across species for cortical (c) and hippocampal (d) pyramidal neurons. P values were calculated by random sampling (see Materials and methods). Sample sizes and abbreviations: brown adipocyte (n = 13, Adi); cardiomyocyte (n = 19, Car); pyramidal neuron, cortex (mouse n = 19, rat n = 8, Pyc); pyramidal neuron, hippocampus (mouse n = 18, rat n = 8, Pyh); serotonergic neuron, dorsal raphe (n = 22, Ser)