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Table 3 Relative allele usage in expression of genes neighboring strain-specific ZFP57 peaks

From: Allele-specific binding of ZFP57 in the epigenetic regulation of imprinted and non-imprinted monoallelic expression

  

Genomic DNA

cDNA ES cells

cDNA NS cells

Gene

Zfp57 peak

BC2

BC8

CB4

CB9

BC2

BC8

CB4

CB9

ANOVA

BC2

BC8

CB4

CB9

ANOVA

B2_Nhlrc1

3′ intergna

38 %

35 %

32 %

38 %

28 %

21 %

38 %

39 %

NS

-

-

-

-

-

B3_Talpid3

Intron

51 %

52 %

51 %

50 %

51 %

51 %

51 %

51 %

NS

54 %

52 %

52 %

53 %

*

B4_Cmtm4

Intron

49 %

52 %

49 %

48 %

54 %

55 %

52 %

52 %

*

47 %

50 %

47 %

49 %

NS

B7_Efhd2

3′ intergn

52 %

53 %

59 %

56 %

37 %

37 %

40 %

38 %

***

53 %

55 %

53 %

57 %

NS

B8_Arhgef10

Intron

42 %

45 %

41 %

40 %

31 %

27 %

26 %

28 %

***

40 %

39 %

39 %

40 %

NS

B10_Fam89

3′ intergn

52 %

48 %

47 %

47 %

84 %

83 %

80 %

87 %

***

93 %

92 %

94 %

88 %

***

B10_Trim67

5′ intergn

45 %

48 %

45 %

46 %

64 %

75 %

84 %

84 %

**

47 %

53 %

47 %

58 %

NS

B12_Nbas

Intron

53 %

44 %

54 %

54 %

66 %

48 %

65 %

66 %

NS

53 %

44 %

54 %

54 %

NS

B13_Ankrd6

Intron

49 %

49 %

47 %

51 %

52 %

51 %

54 %

55 %

NS

41 %

43 %

37 %

44 %

**

B15_Btbd11

Intron

58 %

59 %

59 %

57 %

56 %

57 %

57 %

52 %

NS

53 %

54 %

57 %

57 %

NS

B16_Ccdc11

5′ intergn

51 %

51 %

52 %

53 %

62 %

71 %

62 %

66 %

*

62 %

60 %

62 %

47 %

NS

C1_Zfp553

3′ exon

36 %

35 %

36 %

34 %

73 %

78 %

73 %

75 %

***

39 %

39 %

39 %

43 %

*

C2_Sdk2

Intron

-

-

-

-

12 %

11 %

20 %

20 %

**

59 %

56 %

50 %

56 %

NS

C3_Qrsl1

Exon

50 %

46 %

45 %

48 %

46 %

49 %

51 %

49 %

NS

47 %

48 %

50 %

49 %

NS

C4_Ube2w

Intron

40 %

37 %

39 %

38 %

67 %

66 %

64 %

63 %

***

66 %

66 %

64 %

66 %

***

C5_Usp14

Intron

47 %

45 %

46 %

47 %

47 %

45 %

45 %

46 %

NS

43 %

42 %

43 %

43 %

*

C6_4933422H20Rik

Intron

50 %

48 %

46 %

49 %

21 %

28 %

32 %

36 %

**

8 %

19 %

8 %

22 %

***

C7_Tmem86a

3′ exon

52 %

49 %

52 %

52 %

52 %

41 %

51 %

59 %

NS

46 %

45 %

48 %

44 %

NS

C8_Atrn

Intron

41 %

37 %

37 %

37 %

45 %

42 %

43 %

47 %

*

36 %

38 %

40 %

46 %

NS

  1. a Intergn intergenic ZFP57 binding upstream (5′) or downstream (3′) of the gene. Each row shows the gene name, position of the associated ZFP57 binding site and percentage of BL6 allelic expression ascertained by SNP pyrosequencing (i.e., 100 % = exclusively BL6, 0 % = exclusively Cast and 50 % = perfect biallelic expression). cDNA from four hybrid ES and neural stem cells was analyzed and compared with their corresponding genomic control for PCR bias (gDNA). Values in bold font emphasize significant C57BL/6 or Cast preferential allelic expression (p < 0.01, median difference greater than 15 % compared with genomic DNA control). For Sdk2, where no gDNA is available, ES cells are compared relative to neural stem cell expression. (*p < 0.05, **p < 0.01, ***p < 0.001 repeated measures ANOVA with Bonferroni’s post-test; NS not significant). See also Fig. S10 in Additional file 1 and Fig. 6 highlighting the relative expression level and allelic preference for eight highlighted genes.