Figure 6

Chromatin features underlying TAD and compartment boundaries.(A) Selected profiles for locus-level features are shown for TAD boundaries (CTCF, H3K9me3 and POL2) and compartment boundaries (H2A.Z, H3K4me2 and YY1), as a mean normalized ChIP-seq signal relative to input chromatin per bin (±1 standard error). TAD boundaries were examined over 40-kb bins over the 1 Mb flanking each boundary; compartment boundaries were examined over 100-kb bins over 3 Mb. (B) The significance of enrichment or depletion (− log10P two-tailed Mann–Whitney test) of a feature was calculated as the boundary bin relative to the ten most peripheral bins (five either side). Points are scaled by the absolute mean difference in signal over the boundary relative to the mean of peripheral bins. ChIP-seq, chromatin immunoprecipitation sequencing; TAD, topological domain.