Figure 9

Maternally expressed hb mRNA is stabilized in brat-mutant embryos. Levels of the maternally expressed isoform of hb mRNA (hb-RB) were assayed by RT-qPCR in brat mutant or wild-type embryos collected 0 to 1.5, 1.5 to 3.0, 3.0 to 4.5, and 4.5 to 6.0 h post egg-laying, and normalized to levels of RpL32 mRNA, whose levels are stable throughout this time-course. hb mRNA levels were significantly higher in brat mutant than wild-type embryos at 1.5 to 3.0, 3.0 to 4.5, and 4.5 to 6.0 h (values represent average of three biological replicates +/− standard error of the mean). *P <0.05, **P <0.005 (Student’s t-test).