Analysis of differential gene expression. (A) Reproducibility of ribo-seq experiment under control conditions. (B) Z-score transformation and false discovery rate (FDR) estimation. (C) Heat map diagram of differentially expressed (DE) genes. TE, translation efficiency. (D) DE gene enrichment over KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways. B-H, Benjamini-Hochberg. (E) The magnitude of ribo-seq occupancy alteration during the first 20, 40 and 60 minutes of OGD for the genes detected as DE after 40 minutes. (F) Correlations across replicates of ribo-seq fold changes of genes with >32 reads per time point at different time intervals of OGD exposure. (G) Venn diagrams showing the lack of a relationship between DE genes, mTOR sensitivity and the presence of rHRE.