Figure 1
From: Oxygen and glucose deprivation induces widespread alterations in mRNA translation within 20 minutes
In vitro model of ischemia. (A) Analysis of cellular ATP during OGD. Reduction in ATP becomes significant after 2 h. In the presence of glucose, ATP levels remain unchanged. OD, oxygen deprivation. (B) Decreased intensities of TMRM and J-aggregated form of JC-1 (red) probes under OGD indicate ΔΨm depolarization. (C) Dissimilar to OD, only a minor transient elevation in Epas1 (also known as HIF-2α) levels is seen under OGD (arbitrary units (a.u.)). (D) Characteristic OGD-induced changes in phosphorylation of the proteins involved in regulation of translation. A minor increase in phosphorylated AMPKα under OD suggests a smaller effect of this condition on AMPK signaling. Scale bar is 50 μm. Asterisks demonstrate significant difference. Error bars show standard deviation (A,C,D) or a range in TMRM and JC-1 intensities (B), calculated for 40 to 50 randomly selected cells.