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Table 1 Significant changes between control and ED-treated samples after disturbing G1 germ line imprint erasure - RNA

From: Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming

      % MAT allele Avg. (n = 3)
Transcript Cell type ED Difference (%) SD (%) Student’s t -test P value Imprinted base line (cutoff 80%) Oil Treatment
Nesp MGC VZ -9.1 3.440 0.004 NO (erased) 39 30
Nesp FGC BPA -8.2 4.356 0.042 NO (erased) 38 30
Nesp MGC BPA -7.9 0.956 0.001 NO (erased) 39 31
Asb4 MGC DEHP 8.7 3.769 0.049 NO (erased) 54 63
Zim1 FGC DEHP 9.0 5.406 0.030 NO (erased) 52 61
H19 FGC DEHP 10.6 7.507 0.024 NO (erased) 65 75
Meg3 FGC BPA 13.7 4.713 0.016 NO (erased) 65 79
Meg3 MGC VZ 16.8 4.438 0.002 NO (erased) 73 90
  1. Allele-specific transcription was compared between ED- and vehicle-treated samples for each transcript in 13.5 dpc FGC and MGC using SNuPE assays. Changes in the average (n = 3) allele-specific transcription that were greater than 5% and were statistically significant (P <0.05) were tabulated and ordered according to the difference in maternal allele-specific expression. Baseline allele specificity of transcription in the maternal (MAT) or paternal (PAT) allele was not observed (NO) in the vehicle-treated sample (cutoff 80%), indicating erased imprinting in PGCs. Of those with baseline erasure, the expression or DNA methylation became more biased toward one parental allele in a few instances, indicating a lack of proper erasure of imprinted expression (see the last column).