Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming

Table 1 Significant changes between control and ED-treated samples after disturbing G1 germ line imprint erasure - RNA

Allele-specific transcription was compared between ED- and vehicle-treated samples for each transcript in 13.5 dpc FGC and MGC using SNuPE assays. Changes in the average (n = 3) allele-specific transcription that were greater than 5% and were statistically significant (P <0.05) were tabulated and ordered according to the difference in maternal allele-specific expression. Baseline allele specificity of transcription in the maternal (MAT) or paternal (PAT) allele was not observed (NO) in the vehicle-treated sample (cutoff 80%), indicating erased imprinting in PGCs. Of those with baseline erasure, the expression or DNA methylation became more biased toward one parental allele in a few instances, indicating a lack of proper erasure of imprinted expression (see the last column).