| | | | | |
% MAT allele Avg. (n = 3)
|
|---|
|
Transcript
|
Cell type
|
ED
|
Difference (%)
|
SD (%)
|
Student’s
t
-test
P
value
|
Imprinted base line (cutoff 80%)
|
Oil
|
Treatment
|
|---|
|
Nesp
|
MGC
|
VZ
|
-9.1
|
3.440
|
0.004
|
NO (erased)
|
39
|
30
|
|
Nesp
|
FGC
|
BPA
|
-8.2
|
4.356
|
0.042
|
NO (erased)
|
38
|
30
|
|
Nesp
|
MGC
|
BPA
|
-7.9
|
0.956
|
0.001
|
NO (erased)
|
39
|
31
|
|
Asb4
|
MGC
|
DEHP
|
8.7
|
3.769
|
0.049
|
NO (erased)
|
54
|
63
|
|
Zim1
|
FGC
|
DEHP
|
9.0
|
5.406
|
0.030
|
NO (erased)
|
52
|
61
|
|
H19
|
FGC
|
DEHP
|
10.6
|
7.507
|
0.024
|
NO (erased)
|
65
|
75
|
|
Meg3
|
FGC
|
BPA
|
13.7
|
4.713
|
0.016
|
NO (erased)
|
65
|
79
|
|
Meg3
|
MGC
|
VZ
|
16.8
|
4.438
|
0.002
|
NO (erased)
|
73
|
90
|
- Allele-specific transcription was compared between ED- and vehicle-treated samples for each transcript in 13.5 dpc FGC and MGC using SNuPE assays. Changes in the average (n = 3) allele-specific transcription that were greater than 5% and were statistically significant (P <0.05) were tabulated and ordered according to the difference in maternal allele-specific expression. Baseline allele specificity of transcription in the maternal (MAT) or paternal (PAT) allele was not observed (NO) in the vehicle-treated sample (cutoff 80%), indicating erased imprinting in PGCs. Of those with baseline erasure, the expression or DNA methylation became more biased toward one parental allele in a few instances, indicating a lack of proper erasure of imprinted expression (see the last column).
