Figure 6

Coordinated regulation of GA-related genes by FLC and SVP. (A) Local enrichment of SVP:GFP and FLC proteins bound to genes involved in GA signalling: TEM1, TEM2, DDF1, GA2OX8 and RGL2 (as inferred by ChIP-seq). Bar denotes a 5 kb window. (B) Transcript levels of TEM1, TEM2, DDF1, GA2OX8 and RGL2 in apices of different mutants. Expression was measured by qRT-PCR. (C) Characterization of ga2ox8 mutant alleles. Top: gene model of GA2OX8. The GK-61 F06 insertion site is depicted by a filled triangle. Empty triangles denote CArG-boxes. The grey box indicates the FLC:SVP binding site identified by ChIP-seq. Bottom left: number of leaves at flowering time for WT and two alleles of ga2ox8 (GK-61 F06 and WiscDsLox263B11) measured under LD photoperiod. Bottom right: transcript levels of GA2OX8 in leaves and apices determined in 2-week-old plants by qRT-PCR. (D) Western blot analysis detecting RGA protein levels in apices and leaves of WT and single and double mutants grown for 2 weeks under SDs. (E) Model for FLC and SVP regulating the GA signalling pathway. Direct and indirect regulation described in this study are illustrated as shown. Mean values are accompanied by standard deviation in B and C.