Figure 3

SOX9 mediates invasion and cell cycle arrest. (A) SOX9 knockdown by siRNA in two melanoma cell cultures M080201 and M080310. (B) Boyden chamber assay for siSOX9 knockdown in M080201 and M080310. (C) Representative picture of the Boyden chamber assay for knockdown of SOX9 in M080310. Top panel shows M080310 cells treated with control siRNA (siCtrl). Bottom panel shows M080310 cells treated with siSOX9_1. (D) SOX9 overexpression by lentiviral expression in proliferative phenotype melanoma cell cultures M010817 and M980513. (E) Proliferation assay by EdU pulse in cell cultures transfected with empty vector and pLenti-SOX9. (F) Cell cycle analysis by PI staining for cell cultures transfected with empty vector and pLenti-SOX9 (top and bottom left panels). Quantitation of cell cycle analysis (* = P <0.05, right panel). (G) Boyden chamber assay for SOX9 overexpressing melanoma cell cultures M010817 and M980513 (* = P <0.05). (H) Representative picture of the Boyden chamber assay for SOX9 overexpression in M010817. Top panel shows M010817 transfected with empty vector. Bottom panel shows M010817 transfected with SOX9.