Genomic complexity of
co-occurring fusion genes. (a) Detection by transcriptome sequencing of SOS1-ADCY3 and SND1-CFTR in the H3122 and H2228 EML4-ALK-positive cell-lines, respectively. Schematic representation of the fusion transcripts and some of the transcriptome sequencing reads spanning the fusion point. (b) Top: schematic representation of part of chromosome 2 illustrating the location of ADCY3, ALK, SOS1, and EML4 as well as the relative location of the FISH probes. Bottom, from left to right: ADCY3 break-apart assay, ALK break-apart assay, and simultaneous application of ADCY3 and ALK break-apart assays on metaphase chromosomes of H3122 cells.