Figure 6

Hendra virus induces apoptosis in PaKiT03, but not HEK293T cells. (A) TRAIL-mediated apoptosis pathway. (B) TRAIL relative protein expression in PaKiT03 cells measured by SILAC. (C) mRNA fold change relative to 0 hpi and normalized to GAPDH of TRAIL, TNFRSF10A, TNFRSF11B and CASP13 in PaKiT03 cells at 24, 48 and 72 hpi. (D) Cell viability relative to 0 hpi of PaKiT03 and HEK293T at 24, 48 and 72 hpi with HeV at a multiplicity of infection (MOI) of 5. One-way analysis of variance (ANOVA) was used to compare cell viability between time points. (E) Relative activity of caspase 3/7 normalized to cell viability for PaKiT03 and HEK293T at 0, 24, 48 and 72 hpi with HeV at an MOI of 5. One-way ANOVA was used compared the relative activity of caspase 3/7 between the PaKiT03 and HEK293T cells at each time point. (F) Western blot of PARP1 cleavage at 0, 8 and 24 hpi in HEK293T. The human PARP1 antibody was not cross-reactive with the PaKiT03 cells. *P <0.05, **P <0.01.