Figure 1
From: Characterizing heterogeneity in leukemic cells using single-cell gene expression analysis
t-Distributed stochastic neighbor embedding (t-SNE) analysis of FACS and qPCR single-cell gene expression data. (A) FACS sorting strategy to enrich leukemic and non-leukemic cells. FSC, forward scatter; GFP, green fluorescent protein. (B) t-SNE plot of FACS data for a random sample of 5,000 leukemic and 5,000 non-leukemic single cells. Shading shows the outline of the whole data. (C) t-SNE plot of the qPCR single-cell gene expression data. Each marker represents a single cell. Leukemic cells are represented by triangles and normal cells are represented by circles. CLP, common lymphoid progenitor; CMP, common myeloid progenitor; GMP, granulocyte/monocyte progenitor, HSC, hematopoietic stem cell; MEP, megakaryocyte/erythroid progenitor; MPP, multipotent progenitor.