Figure 5

TFOE expression data mapped onto a a model of MTB metabolism predicts growth restriction. The gene expression from the TFOE dataset was binarized and applied as constraints on simulations using a MTB genome-scale metabolic model [33]. The growth rates of a set of 51 TFOE strains were measured in the presence and absence of TF overexpression. Each bar shows the ratio of growth rates (uninduced/induced) for a given TF, and the strains predicted to have restricted growth are colored red. Of the 10 strains with the largest increase in doubling time, nine were successfully predicted using this approach.