Zygote to ookinete conversion occurs in the presence of transcription inhibitor - translation is essential. (A) Schematic of zygote to ookinete transformation. (B) Representative images of developmental forms observed: retort (left and middle) or mature ookinete (right) cultured for 19 h with 400 mg/mL of α-amanitin (left and middle) or without (right) are labeled with anti-P28 and observed under a fluorescent microscope. Scale bar = 2.5 μm (C) Ookinete conversion rates (% of control) and (D) percentage mature ookinetes in the presence of α-amanitin. (E) Cycloheximide completely arrests post-fertilization development (% of control). Inhibitor concentrations are indicated on the figures as well as incubation times.