Validation of translational repression by in situ GFP-tagging. (A) Four genes were tagged C-terminally with GFP (top panels). These transgene transcripts are detected by RT-PCR in gametocytes. (B) GFP-tagged proteins cannot be detected in blood stage gametocytes, but is strong in ookinetes. (C) Parasite stages are shown by differential interference contrast (DIC), GFP immunofluorescence and Hoechst labeling of the nuclei. Scale bar = 5 μm.