Figure 1
From: Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in the Plasmodium gametocyte
DOZI and CITH-GFP tagged proteins bind known translationally repressed mRNA, but not translating transcripts. Western blots of DOZI::GFP, CITH::GFP, and GFPCON (259.2 reference line that expresses cytoplasmic GFP throughout the life cycle) after IP of Nycodenz-purified gametocytes with anti-GFP (Roche); c-myc; or beads only (top panels). RT-PCR of co-eluted mRNAs (middle and lower panels). p25, p28, and ap2-o which are transcribed in the FG but translated only following fertilization elute with DOZI::GFP and CITH::GFP, while cith and dozi do not. Cytoplasmic GFP does not co-IP any of the shown mRNAs. Cartoons on the left side of the figure illustrate translation (middle panels) or translational silencing (lower panels).