SlEpk1- silenced plants are compromised in resistance elicited by HopQ1-1 recognition in N. benthamiana . (A) N. benthamiana plants, silenced for the genes shown, were vacuum-infiltrated with 5 × 104cfu/ml P. s. pv. tabaci expressing the effector HopQ1-1. Photographs of plants were taken 5 days after infiltration. (B) P. s. tabaci (hopQ1-1) populations in leaves. Silenced plants were infiltrated with 5 × 104cfu/ml P. s. tabaci (hopQ1-1) and sampled to measure bacterial populations. Bars represent the mean of six plants per construct with their corresponding standard error. Asterisks indicate significant differences compared to Ec1-silenced plants using a Student’s t-test (P <0.05). Ec1, Escherichia coli fragment 1 (negative control); NbSAG101 (positive control that compromises ETI).