Validation of spatial expression patterns. Validation of genes found by RNA sequencing using in situ hybridization (first column) combined with immunohistochemistry against Gap43 (third column; overlap of both in column 4). Column 2 shows nuclear staining using DAPI (scale bars represent 100 μm). (A-D) Sox2. (E-H) Couptf1. (I-L) Dclk2. (M-P) Sox11. (Q-T) Maged1. (U-X) Nnat. (Y-B’) Malat1.