Figure 5

Spatial and temporal evolution of CNAs in LGGs and paired recurrent tumors. (A) CNAs in spatially distinct regions of LGGs of 17 patients. CNAs are categorized by detection in all regions (left panel), more than one region but not all regions (middle), or one region (right). Patients are ordered by the number of regions analyzed of each LGG from high to low. (B) Summary of prognostically relevant CNAs in spatially distinct regions and histology. No intratumoral heterogeneity was observed for 1p/19q co-deletion in any of the tumors, while distal 10q loss was often only detected in subclones. OII, oligodendroglioma; AII, astrocytoma; OAII, oligoastrocytoma. (C) CNAs in initial and paired recurrent tumors of 20 patients. CNAs are categorized by detection in initial tumor only (left panel), both initial and recurrence (middle) or detection uniquely in the recurrence (right). Patients are ordered by the histological malignancy grade of the recurrent tumor. (D) Summary of prognostically relevant CNAs in paired initial and recurrent tumors. 1p/19q co-deletion is stable over time, while distal 10q loss surfaces in recurrences, including two with a higher malignancy grade than LGG. Patients are color-coded on the x-axis and chromosomes are ordered on the y-axis, 1 to 22 from bottom to top. Shades of green enable visualization of individual chromosomal arms, their size varying by the number of regions. Hence, a chromosomal arm with many breakpoints based on CNAs is depicted as larger compared with one with fewer breakpoints. CNAs smaller than 5 Mbp were excluded from this figure. Red, copy number loss; blue, copy number gain; black, no CNA. The arrowhead indicates patient 240, the black squares three LGGs analyzed for both spatial and temporal evolution.