Figure 3
From: RRHP: a tag-based approach for 5-hydroxymethylcytosine mapping at single-site resolution
Analysis of RRHP libraries prepared with different DNA input and sequencing depth. (a) Scatter plot of the read counts between RRHP-MspI-1 and RRHP-MspI-3. (b) Scatter plot of the read counts between RRHP-MspI-1 and RRHP-MspI-2. (c) Venn diagrams show RRHP-MspI-1 and RRHP-MspI-3 have 1.45 million 5hmC sites in common while there are 279,259 unique sites in RRHP-MspI-1 and 215,129 unique sites in RRHP-MspI-3. (d) Venn diagrams show RRHP-MspI-1 and RRHP-MspI-2 have 1.3 million sites in common while there are 418,304 unique sites in RRHP-MspI-1 and 231,081 unique sites in RRHP-MspI-3. (e) Box plots shows that 5hmC sites overlapping in RRHP-MspI-1 and RRHP-MspI-3 have higher read counts on average than non-overlapping sites. (f) Box plot shows 5hmC sites overlapping in RRHP-MspI-1 and RRHP-MspI-2 have higher read counts on average than non-overlapping sites. (g) Distribution of read counts of non-overlapping 5hmC sites between RRHP-MspI-1 and RRHP-MspI-3. (h) Distribution of read counts of non-overlapping 5hmC sites between RRHP-MspI-1 and RRHP-MspI-2.